Fig. 3: Correcting misalignment with cryoPROS on PO-HA-Syn and PO-TRPA1 datasets.

a Histograms showing the mean square differences between ground truth projections and estimated reprojections from cryoPROS and conventional autorefine for the PO-HA-Syn dataset. b Pose distributions for TRPA1 and PO-TRPA1 datasets. c Comparison of reconstructed density maps and FSCs between conventional autorefine (top) and cryoPROS (bottom) for the PO-HA-Syn dataset. d Comparison of density maps obtained by reconstruction only (top), autorefine (middle), and cryoPROS (bottom) for the PO-TRPA1 dataset. Density maps in panel (c, d) are overlaid on backgrounds derived from their ground truth atomic models (in magenta). Density map quality metrics for each density map are shown to the right. The global half-maps FSC is plotted as a solid blue line, and the map-to-model FSC is shown as a dotted purple line. The green shaded area (bounded by dotted green lines) indicates the spread of directional resolution values, defined as the mean ±1 standard deviation (SD). The light coral region represents the histogram of directional FSCs.