Fig. 2: HST-NEET infusion products were polyfunctional T cells that recognized multiple HIV antigens.
a HST-NEET products (n = 6) exhibited variable antigen-specific IFN-γ responses against HIV Gag, Pol, Nef, and conserved epitope targets in the Gag/Pol Mos1 and Mos2 peptide mixes. RESIST-004 HST-NEET numbers after treatment-related studies were insufficient to test Gag/Pol Mos1 and Mos2 specificity. Positive ELISpot results were defined as IFN-γ spot forming cells ≥2 times the actin negative control. Spot forming cells (SFC) per 105 cells indicative of specific responses compared to actin stimulated cells: RESIST-001 (actin [17 SFC], nef [139 SFC]), RESIST-003 (actin [19 SFC], gag [659 SFC], pol [466 SFC], nef [1057 SFC], mos1 [540 SFC], mos2 [520 SFC]), RESIST-004 (actin [34 SFC], nef [117 SFC]), RESIST-005 (actin [65 SFC], nef [804 SFC]), RESIST-006 (actin [0 SFC], nef [236 SFC]), and RESIST-007 (actin [25 SFC], gag [1063 SFC], nef [854 SFC], mos1 [291 SFC], mos2 [290 SFC]). b Each bar reports the total sum (infusion 1 and infusion 2) of infused T cells specific for HIV Gag, Pol, and Nef epitopes for each HST-NEETs product (n = 5). c Each bar reports the total sum (infusion 1 and infusion 2) of infused T cells specific for conserved epitopes in Gag/Pol Mosaic 1 and Mosaic 2 immunogens for each HST-NEETs product (n = 5). d, e HST-NEET products (n = 6) were predominantly CD8+ effector memory T cells and had variable expression of T cell surface activation and exhaustion markers. f CD3+ CD8+ HST-NEET products (n = 5) exhibited polyfunctional T cell responses upon stimulation with Gag, Pol, Nef and Gag/Pol Mos1 and Mos2 peptides as measured by intracellular production of IFN-γ and TNF-α. HIV-specific T cell activation was dominant in the CD8+ T cell fraction of all HST-NEET products. Source data are provided as a Source Data file.
