Fig. 3: Epitope specificities of HIV-specific CD8+ T cell responses in HST-NEET products. | Nature Communications

Fig. 3: Epitope specificities of HIV-specific CD8+ T cell responses in HST-NEET products.

From: Autologous HIV-specific T cell therapy targeting conserved epitopes is well-tolerated in six adults with HIV: an open-label, single-arm phase 1 study

Fig. 3

a Comprehensive pooled peptide epitope mapping of HST-NEETs revealed 15-mer peptides driving HIV-specific responses in each product (n = 5). RESIST-001, -003, -005, and -006 ELISpots were conducted as singlets based on HST-NEET numbers available for characterization studies, and RESIST-007 ELISpots were conducted in duplicate. Plotted SFC per 105 cells is shown above each bar. b–f HST-NEET products were stimulated with 15-mer peptides in (a) for 5 h and intracellular production of IFN-γ and TNF-α in CD8+ T cells was evaluated to confirm epitope-specific responses. Peptides evaluated are named by the first two amino acids in the 15-mer sequence followed by the peptide length. g Minimal (9 amino acid) epitopes associated with CD8+ T cell responses were determined for each product. RESIST-004 HST-NEETs were unavailable for these product characterization studies. Positive ELISpot results were defined as IFN-γ spot forming cells ≥2 times the actin negative control. RESIST-005 ELISpots were conducted as singlets, RESIST-001, -003, -006, and -007 were conducted in duplicate. Plotted SFC per 105 cells is shown above each bar.

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