Fig. 4: Differences in HIV-specific T cell responses and anti-Env antibody responses were observed in RESIST-003 and RESIST-006. | Nature Communications

Fig. 4: Differences in HIV-specific T cell responses and anti-Env antibody responses were observed in RESIST-003 and RESIST-006.

From: Autologous HIV-specific T cell therapy targeting conserved epitopes is well-tolerated in six adults with HIV: an open-label, single-arm phase 1 study

Fig. 4

a HIV-specific T cell responses showed significant changes in RESIST-003 post infusion two in response to Gag/Pol Mos2 (p = 0.007), Nef FPVRPQVPL (p = 0.003), Nef RPQVPLRPM (p = 0.002), Mos1/Pol LVGPTPVNI (p = 0.02), Mos1/Pol IIKDYGKQM (p = 0.05) and Mos2/Pol IIRDYGKQM (p = 0.002) peptides. Significant differences were also observed in RESIST-006 post infusion two in response to Gag/Pol Mos2 (p = 0.05) and Nef RPQVPLRPM (p = 0.05) peptides. ELISpots were performed in singlets to assess RESIST-001, RESIST-003, RESIST-004, and RESIST-007 responses due to limited PBMC numbers, and in duplicates for RESIST-005 and RESIST-006 responses. Statistical significance (P-value < 0.05) of IFN-γ T cell responses was compared between grouped SFC values up to and including infusion two, and after infusion two for each HIV antigen as shown in graph legends. b Anti-Env antibody responses were maintained across participant plasma samples with significant differences in antibody responses between pre and post infusion two in RESIST-003 (p = 0.006) and RESIST-006 (p = 0.02). Statistical significance (P-value <0.05) of changes in antibody responses was compared between ED50 values up to and including infusion two, and after infusion two. Blue shaded areas indicate timepoints up to and including the week of infusion two. Each x-axis timepoint has a corresponding y-axis value on the plotted trendlines. Vertical dashed lines (--) indicate infusion one and infusion two, respectively. Two-sided Wilcoxon rank-sum tests were used to determine significant differences between grouped values for timepoints up to and including infusion two, and after infusion two. Bolded p-values in the legends indicate significant differences (p <0.05) between pre and post infusion two responses. Immunological responses were evaluated for all infused participants (n = 6). Summary statistics and source data are reported as a Source Data file.

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