Fig. 2: Induced NUP98::KDM5A degradation causes terminal differentiation of AML blasts.

A Schematic overview of the constructs used for the generation of the dTAG-NUP98::KDM5A cell line. B Representative plots of flow cytometric analysis of intracellular NUP98::KDM5A protein levels upon treatment with dTAG13 or DMSO. (Blue, dTAG-NUP98::KDM5A cell line; grey, untagged NUP98::KDM5A control cell line (Table 1)). C Intracellular flow cytometric analysis of NUP98::KDM5A protein levels after treatment of dTAG-NUP98::KDM5A cells with indicated concentrations of dTAG13 for 18 h (n = 3 biological replicates, mean ± SD, two-sided, unpaired t-test), D and after treatment with 35 nM dTAG13 for indicated time points (n = 3 biological replicates, mean ± SD, two-sided, unpaired t-test). E Flow cytometric analysis of cell cycle distribution of dTAG-NUP98::KDM5A cells after treatment with dTAG13 (35 nM) and DMSO for 3 days (n = 3 biological replicates, mean ± SD, two-sided, unpaired t-test), including representative histogram plots (right). F Flow cytometric analysis of apoptosis after treatment of dTAG-NUP98::KDM5A cells with dTAG13 (35 nM) and DMSO for 3, 7, and 10 days (n = 3 biological replicates, mean ± SD, two-sided, unpaired t-test). G Representative cytospin images showing the morphology of dTAG-NUP98::KDM5A cells after treatment with dTAG13 (35 nM) or DMSO for 3 and 5 days. Scale bar = 20 μm. Representative images of n = 2 samples. H Flow cytometric analysis of surface expression of c-Kit, (I) Gr-1 and (J) Mac-1 of dTAG-NUP98::KDM5A cells after treatment with dTAG13 (35 nM) and DMSO for 7 days (n = 3 biological replicates, mean ± SD, two-sided, unpaired t-test), including representative histogram plots (bottom). Parts of the figure were created in BioRender. Grebien, F. (2025) https://BioRender.com/d32l249. Source data are provided as a Source Data file.