Fig. 2: PDGFRα/β heterodimers dimerize within several minutes.

A Immunoprecipitation (IP) of dimerized PDGFRα/β receptors with GFP-Trap nanobody from cells that were unstimulated or treated with 10 ng/ml PDGF-BB ligand for 2 min to 4 h followed by western blotting (WB) with an anti-PDGFRα or an anti-PDGFRβ antibody. WCL, whole-cell lysates. Images representative of three experiments. Uncropped blots in Source Data. B Line graph depicting quantification of band intensities from n = 3 biological replicates as in A. Data are mean ± s.e.m. P = 0.05 for PDGFRβ 2 min, P = 0.02 for PDGFRβ 5 min (two-tailed, ratio paired t-test). C Immunoprecipitation of dimerized PDGFRα/β receptors with GFP-Trap nanobody from cells that were unstimulated or treated with 10 ng/ml PDGF-BB ligand for 2 min to 4 h followed by western blotting with an anti-phospho (p)-PDGFR antibody. Images representative of three experiments. Uncropped blots in Source Data. D Line graph depicting quantification of band intensities from n = 3 biological replicates as in C. Data are mean ± s.e.m. P = 0.02 for 5 min, P = 0.04 for 15 min, P = 0.01 for 30 min, P = 0.02 for 60 min (two-tailed, ratio paired t-test). Source data are provided as a Source Data file.