Fig. 3: PDGFRα/β heterodimers are rapidly internalized.

A, D, G Scatter dot plots depicting Pearson’s correlation coefficient of the PDGFRαV1/βV2 heterodimer cell line Venus signal with an anti-Na⁺/K⁺-ATPase antibody (A), an anti-RAB5 antibody (D) or an anti-APPL1 antibody (G) signal following 10 ng/ml PDGF-BB ligand stimulation from 1–5 min (A) or 2–30 min (D, G). Data are mean ± s.e.m. P = 0.001 for Na⁺/K⁺-ATPase 1 vs 5 min, P = 0.02 for RAB5 2 vs 10 min, P = 0.04 for RAB5 10 vs 30 min (two-tailed, unpaired t-test with Welch’s correction). Colored circles correspond to independent experiments. Summary statistics from biological replicates consisting of independent experiments (large circles) are superimposed on top of data from all cells; exact number (n) of technical replicates across each of three biological replicates can be found in Source Data. B, C, E, F, H, I Na⁺/K⁺-ATPase antibody signal (white or magenta; B, C), RAB5 antibody signal (white or magenta; E, F) or APPL1 antibody signal (white or magenta; H, I) and/or Venus expression (white or green; B, C, E, F, H, I) as assessed by (immuno)fluorescence analysis of the PDGFRαV1/βV2 heterodimer cell line. Insets in B, C, E, F, H and I are regions where white arrows are pointing. Nuclei were stained with DAPI (blue; B, C, E, F, H, I). White arrows denote colocalization; white outlined arrows denote lack of colocalization. Images representative of three experiments. Scale bars = 20 μm (main images), 3 μm (insets). Source data are provided as a Source Data file.