Fig. 1: Naturally occurring PET hydrolases and their variants do not deviate from the α/β-hydrolase core fold.

Illustrated are selected bacterial polyesterase-lipase-cutinase (PLC)-like^27,34,36,136 and fungal cutinase-like PET hydrolases^42,137,138, alongside phylogenetically distinct enzymes from archaea⁴⁰ and bacteria^27,139  with minimal depolymerization activity but structural uniqueness. These PET hydrolases are grouped based on their C_α root-mean-square deviation (RMSD) relative to LCC as a reference. Ribbon plots are colored with a gradient from low RMSD (blue) to 4 Å and higher (white). The catalytic triad (serine, histidine, and asparagine in all structures) is shown as yellow sticks. PDB codes, RMSD values, and residues included in the calculations are shown below each structure.