Fig. 1: An S-HxxxE motif is critical for yeast toxicity of Ceg14.
From: Modulation of host ATP levels by secreted bacterial effectors
a Sequence alignment of the S-HxxxE motif in proteins from a diverse array of bacterial species. Proteins were identified by PSI-BLAST using Ceg14 as an entry. Information about the bacterial species, accession number of the proteins was shown in the left of the alignment. Highly conserved amino acids were highlighted in red background and residues with similar property were in red. The bacterial species included are: Lp Legionella pneumophila, Rb Rickettsiaceae bacterium, Lb Lentisphaerota bacterium, Vs Vibrio sinus, Pl Photorhabdus luminescens. b The predicted S-HxxxE motif is crucial for Ceg14-induced yeast toxicity. Yeast cells expressing Ceg14 or mutants defective in the S-HxxxE motif from the galactose-inducible promoter were serially diluted and spotted on the specified medium. Images were captured after 2 d incubation at 30 °C (left panel). The expression of Ceg14 and its mutants in yeast was probed by immunoblotting, the metabolic enzyme 3-phosphoglycerate kinase (PGK) was probed as a loading control (right panel). 1, uninduced samples (glucose); 2, induced samples (galactose). Similar results were obtained in three independent experiments. Source data are provided as a Source Data file.
