Fig. 1: Methionine–METTL3–m6A pathway is activated during NPC differentiation.
From: An RNA transmethylation pathway governs kidney nephrogenic potential
A Principal component analysis of the metabolomics data, showing segregation of renewing (rNPCs) and differentiated NPCs (dNPCs) samples (N = 4). B LC/MS-measured methionine and C ELISA-assessed SAM levels in dNPCs compared to rNPCs. D Immunoblot analysis shows the expression of indicated proteins in rNPCs compared to dNPCs. Actin serves as the loading control. Each lane represents pooled lysate from 3 to 4 biological replicate primary NPC cultures. E ELISA (N = 5; biological replicates) and F RNA dot blot analysis showing m6A enrichment in dNPCs compared to rNPCs (N = 3). Methylene blue (MB) serves as a loading control. G–I Images showing MAT2A, METTL3, and m6A immunostaining and DBA labeling in kidney sections from E16.5 embryos (N = 4). Anti-MAT2A, anti-METTL3, and anti-m6A (red); DBA (green); DAPI (blue). Scale bars 25 μm (G–I). Error bars represent the standard error of the mean (SEM). Statistical analysis: two-tailed, unpaired t-test (B, C, and E). Source data are provided as a Source Data file. N indicates biological replicates.
