Fig. 2: SAM and METTL3 mediate NPC fate transition towards differentiation.
From: An RNA transmethylation pathway governs kidney nephrogenic potential
A Immunofluorescence and B immunoblots showing SIX2 and PAX8 expression in NPCs cultured for 48 h in 1.25 μM CHIR NPEM media supplemented with vehicle, 100 μM SAM, 100 μM SAH, or 100 μM SAM and 40 μM METTL3 inhibitor. Actin serves as the loading control. Each lane represents pooled lysate from three biological replicate NPC cultures. C Images showing GFP and PAX8 immunostaining in NPCs treated with different doses of SAM or M3A (METTL3 activator). The average percentages (mean ± SD) of NPCs that are double-positive for GFP and PAX8 are presented in relevant images (N = 3). Source data are provided as a Source Data file.
