Fig. 3: Inhibiting the methionine–METTL3 axis blocks NPC differentiation and nephrogenesis.
From: An RNA transmethylation pathway governs kidney nephrogenic potential
A Immunostaining for PAX8, SIX2, and E-cadherin in E12.5 kidneys cultured in regular or methionine-depleted media supplemented with vehicle, 250 μM SAM, or 100 μM SAH. The number (average ± SD) of PAX8-positive tubules and SIX2-positive NPC niches is depicted in the relevant images. B H&E-stained, SIX2, Pan-CK, and PAX8 immunostained, and DBA-labeled kidney sections from P1 mice of the indicated genotypes. White dotted circles indicate PAX8+ tubules. The graphs depict the number of glomeruli and pre-tubular structures. C PAX8, SIX2, and E-cadherin immunostaining in E12.5 Six2:eGFPTGC/+ (control) and Six2:eGFPTGC/+; Mett3F/F (Six2-Mettl3-KO) kidneys cultured ex vivo for 48 h. Six2-Mettl3-KO kidneys were grown in regular media (vehicle) or media supplemented with 250 μM SAM. Control (wildtype) kidneys were grown in regular media (vehicle) or supplemented with M3i or M3i plus SAM. The number (average ± SD) of PAX8 tubules and SIX2 NPC niches is depicted in relevant images. ** Indicates P < 0.05. Statistical analysis: one-way ANOVA with Tukey’s multiple-comparisons test (A, C); two-tailed, unpaired t-test (B). Error bars represent SEM. Source data are provided as a Source Data file.
