Fig. 4: Activating SAM–METTL3 promotes NPC differentiation and ex vivo nephrogenesis.
From: An RNA transmethylation pathway governs kidney nephrogenic potential
A Kidney sections of E12.5 control or Six2:eGFPTGC/+; Mettl3OE (Mettl3-OE) mice immunostained with SIX2 and pan-cytokeratin, or subjected to in situ hybridization using probes against Cited1 and C1qdc2. B H&E staining, SIX2 (red), and pan-cytokeratin (green) immunostaining of kidney sections from P1 control or Mettl3-OE mice. White arrowheads point to precocious PAX8 expression. C E12.5 kidneys from control, Mettl3-OE, or wildtype embryos were cultured in regular media for 48 h and co-stained for PAX8 and E-cadherin or SIX2 and E-cadherin. The media of wild-type kidneys was supplemented with a vehicle, M3A (100 or 400 μM), or SAM (250 or 1000 μM). The number (average ± SD) of PAX8 tubules and SIX2 NPC niches is depicted in relevant images. ** Indicates P < 0.05. Statistical analysis: One-way ANOVA, Tukey’s multiple comparisons (C). Source data are provided as a Source Data file.
