Fig. 5: Activating SAM–METTL3 promotes in vivo nephrogenesis.
From: An RNA transmethylation pathway governs kidney nephrogenic potential
A Principal component analysis of the metabolites in E16.5 and P3 nephrogenic zone cells (NZCs). B Volcano plot showing differential metabolite abundance between P3 and E16.5, filtered by P value (horizontal dashed line). Methionine and SAM are highlighted as black circles. C LC/MS-measured methionine and SAM levels at P3 and E16.5. D Schematic of the SAM or M3A treatment regimen. Created in BioRender. Patel, V. (2025) https://BioRender.com/6239npyE Kidney-weight-to-body-weight (KW/BW) ratio and F glomerular counts of P10 mice, and G eGFR values of P40 mice treated with vehicle, SAM, or M3A. H Low- and high-magnification images of P4.5 kidneys expressing Six2:eGFP. The number (average ± SD) of GFP clusters per 1 mm² kidney section area is depicted in the relevant images. I Distribution of the number of Six2:eGFP-positive cells within NPC clusters. At least 30 clusters from three biologically independent samples were counted for each condition. J Images of P4.5 kidneys immunostained for PAX8 and GFP are shown. The percentage of PAX8- and GFP-double-positive NPC clusters is indicated. Statistical analysis: Two-tailed, unpaired t-test (C, E–G, I). Error bars indicate SEM; ** indicates P < 0.05. Source data are provided as a Source Data file.
