Fig. 6: LRPPRC is a downstream target of SAM and METTL3.

A Pathway analysis of RNA-Seq datasets showing downregulation of mitochondrial metabolism genes in E16.5 Six2^Cre-Mettl3-KO (M3^KO) compared to control kidneys. B IGV tracks display the input-normalized m⁶A signal on Lrpprc mRNA. C Q-PCR analysis showing Lrpprc (mouse cells) and LRPPRC (human cells) transcripts are enriched in samples recovered after RNA immunoprecipitation using an anti-METTL3 antibody compared to an anti-IgG antibody. N = 3. D Immunoblots showing LRPRRC and oxidative phosphorylation complex expression in Six2^Cre-Mettl3-KO (M3^KO) and Mettl3-OE (M3^OE) kidneys compared to their respective controls. Actin serves as a loading control. E LRPPRC, SIX2, or PAX8 immunostaining, or live-cell MitoTracker labeling of NPCs cultured for 48 h in 1.25 μM CHIR NPEM media and co-treated with 100 μM M3A or 100 μM SAM in the presence or absence of 20 μM GAA. F–H Newborn pups were injected from P1 to P4 with a vehicle, 20 mg/kg GAA, 25 mg/kg SAM, or both 20 mg/kg GAA and 25 mg/kg SAM. Kidney weight-to-body weight (KW/BW) ratio (F) and glomerular count (G) of P10 mice, and eGFR (H) of P40 mice, are shown. I–K Newborn pups were injected daily from P1 to P4 with a vehicle, 20 mg/kg GAA, 2.17 mg/kg M3A, or both 20 mg/kg GAA and 2.17 mg/kg M3A. KW/BW ratio (I) and glomerular count (J) of P10 mice, and eGFR (K) of P40 mice are shown. Statistical analysis: Fisher’s Exact Test (A), two-tailed unpaired t-test (C), and one-way ANOVA with Tukey’s multiple comparisons (F–K); error bars indicate SEM. Source data are provided as a Source Data file.