Fig. 1: MAVS deficiency protects hearts from MIRI in vivo. | Nature Communications

Fig. 1: MAVS deficiency protects hearts from MIRI in vivo.

From: Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice

Fig. 1

a MAVS expression in remote area, area at risk and infarct area of WT hearts in sham vs 6-hour reperfusion mice. b Statistical analyses in (a). c MAVS expression in mitochondrial preparations of HL-1 cells after 6 h hypoxia and 8 h reoxygenation, with mitochondrial cytochrome coxidase IV (COX-IV) as the mitochondrial internal reference. d Statistical analyses in (c). e Echocardiography of WT vs MAVS-KO mice at 6 h reperfusion. Yellow line shows the left ventricle anterior and posterior wall position, white double-tailed arrow shows the inside diameter. LVIDS is left ventricular internal diameter at end systole, LVIDD at end diastole. Scale bar, 1 mm. f Cardiac contractility parameters of WT vs MAVS-KO mice at 6 h of reperfusion. Left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDV). n = 10. g 2, 3, 5-triphenyltetrazolium chloride + Evans blue staining of WT vs MAVS-KO mice at 1 day of reperfusion. Scale bar, 2 mm. h Infarct volume ratio from staining in (g). n = 8. i Pathophysiological characteristics by hematoxylin-eosin staining in WT vs MAVS - KO mice at 3 days reperfusion. Black line shows the infarct area. Scale bar, 1 mm. j Infarct size quantification in i. n = 5. k Ultrastructure of heart infarct area in WT vs MAVS-KO mice at 6 h reperfusion by transmission electron microscopy. Red arrows indicate damaged mitochondria. Scale bar, 500 nm. l Fold change of damaged mitochondria in k. n = 8. m Terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling (TUNEL) staining in WT vs MAVS-KO heart sections at 6 h after reperfusion. Wheat germ agglutinin (WGA) stains cardiomyocytes. Scale bar, 50 μm. n Apoptotic cell quantification in (m). n = 5 mice. o Serum cardiac enzymes levels at 6 h reperfusion or sham. n = 5 mice. b, d Data from 3 independent samples are presented as means ± SD. Statistical tests used: unpaired 2-tailed Student t test for (d, h, j, l, n); one-way ANOVA with Tukey’s test for (b, f, o). Significance levels: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns (p ≥ 0.05). Source data are in the Source Data file.

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