Fig. 2: Myocardium-specific MAVS knockdown phenotype mirrors the heart phenotype of global MAVS knockout during MIRI. | Nature Communications

Fig. 2: Myocardium-specific MAVS knockdown phenotype mirrors the heart phenotype of global MAVS knockout during MIRI.

From: Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice

Fig. 2

a Echocardiography of MAVS knockdown (MAVS-sh) and negative control knockdown (NC-sh) mice at 6 h reperfusion. Yellow line shows the left ventricle anterior and posterior wall position, white double-tailed arrow shows the inside diameter. LVIDS, Left ventricular internal diameter end systole; LVIDD, Left ventricular internal diameter end diastole. Scale bar, 1 mm. b Cardiac left ventricular ejection fraction (LVEF), left ventricular fraction shortening (LVFS), left ventricular end-systolic volume (LVESV) and left ventricular end-diastolic volume (LVEDV) in MAVS-sh and NC-sh mice at 6 h reperfusion or sham. n = 10 mice per group. c Infarct volume in left ventricle based on 2, 3, 5-triphenyltetrazolium chloride + evans blue staining. n = 7 mice per group. d Pathophysiological features by hematoxylin-eosin staining in MAVS-sh mice and NC-sh mice at 3 days reperfusion. The black line shows the infarct area. Scale bar, 1 mm. e Infarct sizes quantification in (d). n = 7. Data are shown with 7 biologically independent samples. f Terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling (TUNEL) staining in heart sections of MAVS-sh and NC-sh mice at 3 days reperfusion. Scale bar, 50 μm. g Apoptotic cell percentage in intact 4’,6-diamidino-2-phenylindole (DAPI) positive cells in (f). n = 5 mice. h Ultrastructure of infarct area at 6 h reperfusion by transmission electron microscope. Red arrows indicate damaged mitochondria. Scale bar, 500 nm. i Damaged mitochondria percentage quantified in MAVS-sh and NC-sh mice. n = 10 mice in each group. j Cardiac enzymes detection in the serum at 6 h reperfusion or sham. n = 5 mice. k Reactive oxygen species in cardiac tissue at 6 h reperfusion or sham. n = 5 mice. Data are means ± standard deviation; statistical analyses were conducted by unpaired 2-tailed Student t test (c, e, g, i) and one-way analysis of variance with Tukey’s multiple comparisons test (b, j, k); *p < 0.05, **p < 0.01, ***p < 0.001; ****p < 0.0001, ns, p ≥ 0.05. Source data are provided as a Source Data file.

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