Fig. 3: Protein-protein interactions between YoaA-χ and SSB are necessary for YoaA-χ to unwind SSB-bound DNA.
From: The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA
Representative time courses for DNA unwinding of 65-nt 5ˈ overhang DNA (50 nM, O1) are shown for reactions with. A no SSB and 2, 5, 10, 20, and 50 nM of YoaA-χ R128A. B wt SSB (75 nM) prebound to DNA and 2, 5, 10, 20, and 50 nM of YoaA-χ R128A. C SSB ΔC1 (75 nM) prebound to DNA and 2, 5, 10, 20, and 50 nM of YoaA-χ. D SSB ΔC1 (75 nM) prebound to DNA and 2, 5, 10, 20, and 50 nM of DinG. All experiments were performed in triplicate. Darkness in blue corresponds to increasing concentrations of helicase. On the DNA schematic, the red circle indicates Cy5, the yellow circle indicates Cy3, YoaA-χ is denoted in blue, SSB in green, DinG in orange, and pink stars represent protein mutations. Source data are provided as a source data file.
