Nature Communications

Table 1 Tension and ATP concentration dependencies of helicase activity

From: Autoregulation of the real-time kinetics of the human mitochondrial replicative helicase

		Tension		[ATP]
		No mtSSB	With mtSSB	No mtSSB
Average unwound bp	WT	37 ± 6 ^L	128 ± 14^L	49 ± 8⁽¹⁾
	WT	19 ± 1^H	37 ± 9^H	44 ± 5⁽¹⁰⁾
	ΔZBD	77 ± 29^L	84 ± 8^L	27 ± 6⁽¹⁾
	ΔZBD	28 ± 3^H	27 ± 4^H	44 ± 5⁽¹⁰⁾
	ΔC-tail	35 ± 6^L	41 ± 5^L	32 ± 6⁽¹⁾
	ΔC-tail	29 ± 7^H	26 ± 7^H	39 ± 7⁽¹⁰⁾
Average velocity(bp/s)	WT	0.8 ± 0.2^L	2.4 ± 0.5^L	0.7 ± 0.2⁽¹⁾
	WT	2.4 ± 0.4^H	0.8 ± 0.1^H	3.6 ± 0.6⁽¹⁰⁾
	ΔZBD	2.8 ± 1.1^L	2.3 ± 0.3^L	1.5 ± 0.3⁽¹⁾
	ΔZBD	2.4 ± 0.3^H	2.8 ± 0.3^H	4.3 ± 0.2⁽¹⁰⁾
	ΔC-tail	3.7 ± 0.9^L	4.1 ± 0.7^L	3.4 ± 0.6⁽¹⁾
	ΔC-tail	2.9 ± 0.9^H	3.1 ± 1.2^H	5.0 ± 1.4⁽¹⁰⁾
Pause occupancy	WT	0.86 ± 0.03^L	0.72 ± 0.05^L	0.90 ± 0.08⁽¹⁾
	WT	0.69 ± 0.08^H	0.84 ± 0.07^H	0.62 ± 0.05⁽¹⁰⁾
	ΔZBD	0.75 ± 0.08^L	0.70 ± 0.04^L	0.80 ± 0.06⁽¹⁾
	ΔZBD	0.73 ± 0.09^H	0.67 ± 0.05^H	0.50 ± 0.06⁽¹⁰⁾
	ΔC-tail	0.59 ± 0.09^L	0.59 ± 0.06^L	0.62 ± 0.08⁽¹⁾
	ΔC-tail	0.60 ± 0.08^H	0.67 ± 0.07^H	0.60 ± 0.07⁽¹⁰⁾

Tension dependencies were measured at 4 mM ATP. Superscripts L and H denote values at lowest and highest tensions tested, respectively, for each helicase. ATP concentration dependencies were measured at 6 pN. Superscripts (1) and (10) denote values at 1 and 10 mM ATP, respectively. Error values represent s.e. Sample sizes (N): Tension dependencies without mtSSB: WT N = 45; ΔZBD N = 32; ΔC-tail N = 27. Tension dependencies with mtSSB: WT N = 57; ΔZBD N = 29; ΔC-tail N = 18. ATP concentration dependencies: WT N = 45; ΔZBD N = 45; ΔC-tail N = 36.
The table summarized the average unwound nucleotides, unwinding velocities (bp/s), and pause occupancies of the studied helicases under varying tension and ATP concentrations ([ATP]).

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