Fig. 6: Methylated R10 is more insensitive to ABA than demethylated R10.

a Germination phenotypes of WT and R10-GFP, R10^3K-GFP, and R10^3F-GFP overexpressing lines treated with different concentrations of ABA. Photographed on the seventh day. Bars, 1 cm. Germination time course for WT and R10-GFP, R10^3K-GFP, and R10^3F-GFP overexpressing lines on ½ MS medium supplemented with 0 μM (b), 1 μM (c), or 2 μM (d) ABA. Data are means ± SD (n = 18 seedlings). Heights (e) and root lengths (f) of 5-day-old WT, and R10-GFP, R10^3K-GFP, and R10^3F-GFP overexpressing lines grown on ½ MS. Data are means ± SD (n = 17 seedlings). Three biological replicates were used for each treatment in a–d. All above P values were calculated by a two-tailed t-test. All descriptive statistical values for all box plots and source data are provided as a Source Data file in a–f.