Fig. 8: OsPRMT6b promotes the decline of ABA signal after high-temperature stress.

a OsPRMT6b and R10 protein levels in WT and osprmt6b-1 mutant seedlings grown in the field. 60-day-old seedlings were collected for protein extractions at the indicated time points during the day. Numbers below the bands show the relative protein levels of OsPRMT6b/HSP82 or R10/HSP82 (n = three independent experiments). The hour numbers represent daytime hours, and the corresponding temperatures refer to the temperature achieved at that moment. b OsPRMT6b and R10 protein levels in WT and osprmt6b-1 mutant seedlings grown in the climate chamber. WT and osprmt6b-1 seedlings were grown in a normal growth chamber (10 h darkness, 25 °C/14 h light, 30 °C conditions, light intensity of 500–800 μmol·m⁻²·s⁻¹ and relative humidity of 70%) for 14 days and then placed in a simulated high-temperature growth chamber with continuous light for 2 days (n = three independent experiments). Samples were taken at the indicated times. Numbers below the bands show the relative protein levels of OsPRMT6b/HSP82 or R10/HSP82. The hour numbers represent daytime hours, and the corresponding temperatures refer to the temperature achieved at that moment. c Plant heights of WT and osprmt6b-1 seedlings grown in mock and after high temperature conditions. One-week-old WT and osprmt6b-1 seedlings grown in a simulated natural long-day growth chamber were transferred into a simulated high-temperature growth chamber for 5 days prior to recording plant height. Three biological replicates were used for each treatment. Data are means ± SD (n = 17 plants). Regression analysis was conducted on the data of plant heights. The slope represents the change in value. All descriptive statistical values for all box plots are provided in the Source Data files. d Relative changes in plant height for c. Data are means ± SD (n = 17 plants). All above P values were calculated by a two-tailed t-test. Source data are provided as a Source Data file in a–c.