Fig. 6: In vivo distribution of Trojanbots.
From: A self-directed Trojanbot-enzymatic nanobot in neutrobot for active target therapy of glioblastoma
a The schematic representation of the construction of the brain glioma model. b In vivo fluorescence images of orthotopic GL261-Luc mouse models with GBM tumors following a single tail vein injection of potassium fluorescein (n = 3 mice per group). c Fluorescence intensity measurements of glioma mouse brains treated with various therapeutic agents (n = 3 mice per group). d Fluorescent images (IVIS imaging) of the brain and major organs, taken 48 h after injection (n = 3 mice per group). e Quantitative ROI analysis of DiR signals in the brain and organs (n = 3 mice per group) (exact P value: Spleen CatNbot vs. Trojanbot P = 5.89774E−5, Lung EMV@GeNP vs. Trojanbot P = 3.86669E−5, Brain EMV@GeNP vs. Trojanbot P = 3.98E−8, Brain CatNbot vs. Trojanbot P = 1.084E−7, NE@EDGN vs. Trojanbot P = 1.62003E−5). f Fluorescence images (IVIS imaging) of brain slices from four groups of mice, labeled with DiR (8 h post tail vein injection). g Quantitative analysis of the fluorescence images (IVIS imaging) of brain slices from the four groups (n = 3 mice per group). (exact P value: EMV@GeNP vs. Trojanbot P = 6.055E−7). h Representative fluorescence images of glioma tissue slices from GBM mice. In the images, EMV@GeNPs, CatNbots, EMV@GeNPs within NE@EDGN, and CatNbots within Trojanbots were labeled with FITC (green), while DOX exhibits intrinsic autofluorescence properties (red). The nuclei of the GL261 cells were stained with Hoechst 33342 (blue). Data are presented as mean ± SD of three independent experiments (e, g). P-values are determined by one-way ANOVA and Tukey multiple comparison tests in (e, g). Source data are provided as a Source Data file.
