Fig. 4: PG filopodia contain the machinery involved in ecdysone secretion. | Nature Communications

Fig. 4: PG filopodia contain the machinery involved in ecdysone secretion.

From: Filopodia are essential for steroid release

Fig. 4

Zoomed-in images of a PLC-GFP-expressing clone showing that CSP (a) and Syx1A (b) localize in filopodia in the sub-BM area. Arrowheads indicate CSP and Syx1A puncta within the filopodia. c A Ypet-Atet-expressing clone showing Ypet-Atet at the membrane and in filopodia (arrowheads) at the sub-BM region. Zoomed-in images of Ypet-Atet-expressing clones showing CSP (d, arrowheads) and Syx1A (e, arrowheads) dots in filopodia. f A clone expressing Syt1-GFP where Syt1-GFP localized at the membrane and in filopodia (arrowheads). g ex vivo tracking of a Syt1-GFP vesicle (arrowhead) in a PG filopodium over time. Note that Ypet-Atet and Syt1-GFP were overexpressed for a short period of time (7 h) to achieve low protein expression levels. White scale bars: 20 μm; yellow scale bars: 5 μm. For each result shown here, n ≥ 3.

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