Table 2 Percentages of identified sterols in the meltwater ponds, lagoon, and in the relict microbial mat

From: Biosignatures of diverse eukaryotic life from a Snowball Earth analogue environment in Antarctica

Peak

RRT

Systematic name

Possible sterol sources

Skua

New

Duet

Fresh

Fogg

Roger

Cono

Orange

Casten

AMS

Legin

P70

Brack

Salt

Lagoon

Relict

1

0.957

22-trans-24-norcholesta-5,22E-dien-3β-ol

Diatoms31,34,38; Dinoflagellates38

            

3.2

   

2

0.970

5α-cholest-22E-en-3β-ol

Dinoflagellates35

1.3

               

3

0.979

5β-cholestan-3β-ol

Diagenesis of cholest-5-en-3β-ol

6.2

          

2.2

   

7.5

4

0.982

Unknown Steroid

n.a.

1.5

               

5

0.988

27-nor-(24S)-cholesta-5,22E-dien-3β-ol

Diatoms31,32,33; Microalgae32,37

        

0.2

 

0.3

0.3

3.3

1.8

  

6

0.990

Unknown Steroid

n.a.

2.4

               

7

0.993

cholesta-5,22E-dien-3β-ol

Diatoms23,31,32; Red algae23,40; Sponges36

2.6

2.7

2.1

1.5

1.9

 

2.0

2.1

5.1

 

2.3

 

13.0

4.7

 

1.7

8

0.996

Unknown Steroid

n.a.

0.6

           

0.4

   
 

0.997

Unknown Steroid

n.a.

0.6

               

10

1.000

cholest-5-en-3β -ol(2H7)

n.a.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

n.i.

11

1.001

cholest-5-en-3β-ol

Animals23; Fungi23; Microalgae23,32; Diatoms30,31,32; Red algae32; Green algae32; Dinoflagellates32,34

15.0

18.2

25.7

21.8

11.5

33.7

17.2

18.7

19.5

38.6

24.8

19.6

16.7

21.8

26.5

17.3

12

1.006

5α-cholestan-3β-ol

Diagenesis of cholest-5-en-3β-ol

5.0

2.5

3.3

3.6

1.2

 

3.3

0.9

2.3

 

3.1

3.4

2.2

2.1

10.7

7.1

13

1.008

Unknown Steroid

n.a.

0.7

               

14

1.012

24-methylcholesta-5,22E-dien-3β-ol

Diatoms30,31,34,39; Cercozoa32; Dinoflagellates35; Microalgae23

10.8

14.6

4.4

8.7

5.4

15.5

6.6

7.1

8.8

12.7

12.0

9.2

20.5

34.0

18.8

17.8

15

1.018

5α-cholest-7-en-3β-ol

Diatoms33,34

5.5

11.8

4.5

7.2

0.9

 

1.3

trace

5.9

 

2.9

2.8

trace

   

16

1.026

24-methylcholesta-5,24(28)-dien-3β-ol

Diatoms31,34; Sponges36

 

8.4

5.0

6.6

14.4

 

8.9

6.1

6.1

 

7.8

11.4

9.1

14.9

 

5.6

17

1.026

(24R)-ethyl-5β-cholestan-3β-ol

Diagenesis of (24S)-ethylcholesta-5,22E-dien-3β-ol

5.4

co.e.

co.e.

co.e

    

co.e.

  

co.e.

    

18

1.028

(24R)-methylcholest-5-en-3β-ol

Diatoms31,32; Green algae32,40

7.2

9.6

6.2

8.5

18.4

16.9

7.0

8.4

15.6

11.8

7.0

7.9

7.1

2.8

10.2

7.4

19

1.030

(24R)-methyl-5α-cholestan-3β-ol

Diagenesis of (24R)-methylcholest-5-en-3b-ol

2.2

 

1.2

1.7

  

1.4

 

1.3

 

0.8

1.5

1.1

   

20

1.035

(24S)-ethylcholesta-5,22E-dien-3β-ol

Diatoms31,34,41; Cercozoa32; Microalgae23,32

7.0

4.1

6.4

8.7

13.2

10.1

5.3

7.5

7.7

11.8

6.6

6.5

8.8

4.0

10.6

13.8

21

1.037

23,24-dimethyl-5α-cholest-22E-en-3β-ol

Diatoms23,34; Dinoflagellates35,36

2.1

 

3.0

   

1.3

   

1.1

2.0

    

21

1.037

Unknown Compound

n.a.

co.e.

 

co.e.

   

co.e.

   

co.e.

co.e.

    

22

1.043

24-methyl-5α-cholest-7-en-3β-ol

Green Algae32; Diatoms34,39; Fungi23

1.5

3.2

2.3

2.9

3.8

 

4.4

10.9

2.2

 

trace

2.8

2.6

   

23

1.045

Unknown Steroid

n.a.

0.6

0.2

0.9

trace

    

trace

 

1.0

0.8

0.9

1.2

  

24

1.048

(24R)-ethylcholest-5-en-3β-ol

Diatoms31,34,39; Green algae32,41

15.7

14.1

24.6

16.2

23.3

23.8

35.4

27.6

16.0

25.1

25.6

18.3

10.4

11.7

23.2

22.0

25

1.051

(24R)-ethyl-5α-cholestan-3β-ol

Diagenesis of (24R)-ethylcholest-5-en-3β-ol and (24S)-ethylcholesta-5,22E-dien-3β-ol

4.0

3.4

3.4

4.1

3.6

 

2.6

1.6

3.0

 

2.1

8.0

0.1

0.9

  

25

1.051

24-ethylcholesta-5,24(28)Z-dien-3β-ol

Algae32,36,40; Sponges36; Green algae23,32,41; Diatoms33

co.e.

      

co.e.

     

co.e.

  

26

1.057

4α,23,24-trimethylcholest-22E-en-3β-ol

Dinoflagellates23,36,38; Diatoms30,31,39

0.9

               

26

1.057

See table description

n.a.

co.e.

               

27

1.060

Unknown Steroid

n.a.

       

3.7

        

28

1.064

(24S)-ethyl-5α-cholesta-7,22E-dien-3β-ol

Green algae40; Microalgae32

1.1

3.5

3.3

2.3

2.4

 

3.2

5.5

1.9

 

1.1

1.5

0.6

   

29

1.069

Unknown Steroid

n.a.

   

2.3

            

30

1.072

Unknown Steroid

n.a.

 

3.7

3.7

4.0

  

trace

 

4.3

 

1.4

1.7

    
  1. Cholest-5-en-3β-ol(2H7) is not included (n.i.) in the steroid abundances. Repeat peak numbers indicate identification of clear co-elution between the first compound and the second, noted by co.e. where appropriate. Certain names are abbreviated: Fogg is short for Fogghorne, Cono is short for Conophyton, Casten is short for Castenholz, and P70 is short for Pond Seventy. The systematic name for peak 26 is 2,5,7,8-tetramethyl-2-(4,8,12-trimethyltridecyl)−6-chromanol. Common names for sterols are located in Supplementary Table 1.