Fig. 2: Neutralizing antibody levels against H5 influenza pseudoviruses and H5N1 vaccine strain A/Vietnam/1194/2004 before and after vaccination, and correlation analysis among hemagglutination inhibition (HI), microneutralization (MN), and pseudovirus-based neutralizing assays (PBNA).
Shown are the ID50 values representing neutralizing antibody levels in human sera before and after vaccination against HA of various H5 strains, as measured by PBNA. Based on post-vaccination neutralizing antibody levels against the vaccine strain, subjects were classified into high, medium, and low response groups, as represented in (a, b, c). Orange dots represent pre-vaccination, while light blue dots represent post-vaccination. The thick black line indicates the pre-vaccination geometric mean titer adding a 95% confidence interval. Geometric mean increase (GMI) and seroconversion rates (SCR) between pre- and post-vaccination are shown above each group. The strains were classified into clades in (c). d, e Pearson correlation analysis (two-tailed) was used to evaluate the association between PBNA and HI or MN. f shows the relationship between PBNA and overall neutralizing antibody SCR, with simple linear regression equation. Statistical significance was assessed using multiple unpaired two-sided t tests, assuming individual variances for each row. The False Discovery Rate was controlled at 1% using the two-stage step-up method of Benjamini, Krieger, and Yekutieli. Source data are provided as a source data file.
