Fig. 1: Putative chromatin remodeling ATPases in Toxoplasma.

a Domain structures of the putative chromatin remodeling ATPases in Toxoplasma identified by BLAST searches. DEXDc: DEAD-like helicases superfamily (SMART #: SM00487); HELICc: helicase superfamily c-terminal domain (SMART #: SM00490); SANT: SANT SWI3, ADA2, N-CoR and TFIIIB” DNA-binding domains (SMART #: SM00717); AT_hook domain: DNA binding domain with preference for A/T rich regions (SMART # SM000384); SnAC: Snf2-ATP coupling, chromatin remodeling complex (SMART #: SM01314); zf-PARP: Poly(ADP-ribose) polymerase and DNA-Ligase Zn-finger region (SMART #: SM01336); RING: Ring finger (SMART #: SM00184); UBA_4: UBA-like domain (Pfam #: PF14555); COM: Chromatin organization modifier domain (SMART #: SM000298); DUF: DUF4208 domain (SMART #: SM001176); PHD: PHD zinc finger (SMART #: SM000249); BROMO: bromo domain (SMART #: SM000297); SNF2_N: SNF2_N domain (Pfam #: PF00176). b Phylogenetic analyses of the putative chromatin remodeling ATPases in Toxoplasma, which were done in MEGA 11 using the maximum likelihood algorithm. c IFA checking the localization of putative chromatin remodeling ATPases in Toxoplasma, as well as the depletion of their expression by IAA treatments, using strains with each of the ATPases tagged with an mAID degron (which contains an HA tag) at their C-termini in the endogenous gene loci. d Impact of chromatin remodeling ATPases depletion on parasite growth, as determined by plaque assays using mAID tagged strains with or without IAA treatment. The parental strain ME49-Tir1 was included as a control. e Impact of the depletion of chromatin remodeling ATPases on parasite division. All patterns that are different from the regular endodyogeny are classified as “Abnormal”. Means ± SD of n = 3 independent experiments, ****p < 0.0001, two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.