Fig. 4: SNF2L depletion results in expression changes of genes involved in parasite development.

a Venn diagram showing the overlap of genes upregulated (≥2-fold) in the SNF2L-depleted mutants with the genes that are upregulated in wildtype merozoites (Mz), bradyzoites (Bz), or sporozoites (Sp) compared with tachyzoites (Tz). Data for the latter are from published datasets available in ToxoDB. ***p < 0.0001, Pearson’s Chi-squared tests indicating genes upregulated in the SNF2L depletion mutant were significantly enriched in gene sets associated with bradyzoites, merozoites, or sporozoites. b For the 301 genes that were upregulated in the SNF2L-depleted mutants, their expression patters in wildtype strain during the life cycle were plotted as a heatmap using data from ToxoDB. c Life cycle expression patterns of the top 30 upregulated genes (excluding hypothetical proteins) in the SNF2L-depleted mutants were plotted as a heatmap. d expression patterns HAP2, PF16, and TGME49_30633839 during the parasites’ life cycle, as well as in iSNF2L mutants with or without IAA treatment. e Volcano plot showing the abundance change of proteins in the iSNF2L strain before and after IAA treatment for 12 h, as determined by TMT-based quantitative proteomics. Data from three biological replicates were plotted. Raw data are shown in Supplementary data 3. f Expression of PF16 in the SNF2L-depleted mutant, as determined by IFA on the iSNF2L/PF16-Ty strain with or without IAA treatment for 60 h. Source data are provided as a Source Data file.