Fig. 5: SNF2L binds to the promoter regions of target genes and its depletion alters chromatin accessibility.

a Enrichment of SNF2L near the transcription start site (TSS) (from −3 Kb to 3 Kb region centered around TSS), as determined by CUT&Tag in the SNF2L-3HA strain using an HA antibody. Pull-down with a naive mouse IgG was included as a control. b Genome browser view of the position and intensity of DNA sequences bound by SNF2L. Data were derived from (a) and similar CUT&Tag experiments done in the iSNF2L strain with or without 12 h IAA treatment. Chromosome XII was shown as an example. c Distribution of SNF2L on different types of genetic elements, derived from the analysis of data in (a). d Enrichment of SNF2L at the upstream regions of selected genes, using data from (a). Meanwhile, the accessibility of chromatin revealed by ATAC-Seq at these regions in the iSNF2L strain treated with or without IAA was also included for comparison. e, f Overall chromatin accessibility in the iSNF2L strain treated with or without IAA, as determined by ATAC-Seq (e). Chromosome XII was shown as an example (f). g accessibility of different types of genetic elements in the iSNF2L strain with and without IAA treatment, derived from the analysis of data in (e). h accessibility of the upstream regions of genes that are upregulated (upper panel) or downregulated (lower panel) in the SNF2L-depleted parasites. Means ± SEM were plotted. Source data are provided as a Source Data file.