Fig. 6: SNF2L interacts with novel nuclear factors to form a gene regulation complex. | Nature Communications

Fig. 6: SNF2L interacts with novel nuclear factors to form a gene regulation complex.

From: Regulation of the developmental programs in Toxoplasma by a novel SNF2L-containing chromatin remodeling complex

Fig. 6

a–d Protein hits from co-IP experiments that were used to identify binding partners for the prey proteins (marked in red). The number of unique peptides derived from untagged (Ctrl) and HA tagged (HA) strains was used to generate these graphs. e co-IP results analyzed by the Straightforward Filtering IndeX (SFINX) to show the possible protein interactions in the SNF2L-containing complex. Raw data are shown in Supplementary data 5. f–h co-IP and Western blotting examining the interactions between SNF2L, SLIF1, and SLIF2. i Cartoons showing the SNF2L/ISW2 chromatin remodeling complexes in indicated organisms. j–l Heat maps showing the life cycle expression patterns of genes upregulated (≥2-fold) in SLIF1, SLIF2, or AP2X-4 depleted mutants. Data were derived from ToxoDB. m Venn diagram showing the overlap of genes upregulated (≥2-fold) in SNF2L, AP2X-4, and SLIF1 depleted mutants. Source data are provided as a Source Data file.

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