Fig. 7: T cell repopulation following radiation-induced TIL depletion is driven by returning high-frequency clonotypes and newly infiltrating singletons, globally independent of TCR sequencing patterns. | Nature Communications

Fig. 7: T cell repopulation following radiation-induced TIL depletion is driven by returning high-frequency clonotypes and newly infiltrating singletons, globally independent of TCR sequencing patterns.

From: Radiation therapy results in preferential tumor antigen-specific lymphodepletion in head and neck cancer

Fig. 7

a Radiation-induced TIL depletion is associated with a major reduction in overall TCR clonotype diversity, which begins to return by 6 weeks post-radiation in all patients. b Clonotype frequency mapping reveals broad immediate TIL depletion with return of high-frequency and intermediate-frequency clonotypes by 6 weeks post-radiation. c Despite alterations in overall number of clonotypes at each timepoint, diversity is conserved on the amino acid-level at the CDR3 region. d Among all repopulating clonotypes approximately 30% are returning clones while the remaining are newly infiltrating. e Clonal tracking of the 30 most frequent clonotypes within pre-treatment tumors reveals that returning clonotypes are predominantly composed of pre-treatment expanded populations.

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