Fig. 2: ELD1 is broadly expressed and encodes a nuclear speckle localized protein. | Nature Communications

Fig. 2: ELD1 is broadly expressed and encodes a nuclear speckle localized protein.

From: ELD1 mediates photoperiodic flowering via OsCCA1 alternative splicing and interacts with phytochrome signaling in rice

Fig. 2

a Rhythmic expression of ELD1 over 24 h in WT and eld1 mutant, detected by RT-qPCR in the leaves of 40-day-old plants grown under controlled long-day conditions (CLD, 14 h light/10 h dark). Values are means ± SD (n = 3 biological replicates). b RT-qPCR analysis of ELD1 expression in various tissues at heading stage. Values are means ± SD (n = 3 biological replicates). c GUS staining of the tissues collected from pELD1:GUS transgenic plants at the heading stage. (1) young panicle, (2) (3) panicle, (4) flag leaf, (5) stem node, (6) root, and (7) stem. Scale bars, 2.5 mm. d Subcellular localization of ELD1-GFP and free GFP in root cells of 7-day-old transgenic plants. Scale bars, 10 μm. e Subcellular localization of full-length ELD1 and various truncated forms in rice protoplasts. ELD1 (1–377) represents the full-length ELD1, ELD1-N (1–81) includes the conserved N-terminal, ELD1-M (82–205) contains the conserved ZCCHC domain, and ELD1-C (206–377) comprises the RS-enriched C-terminal region. D53-mCherry was used as a nuclear marker. Scale bars, 5 μm.

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