Fig. 5: The function of super-enhancers in muscle fiber-type transformation. | Nature Communications

Fig. 5: The function of super-enhancers in muscle fiber-type transformation.

From: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation

Fig. 5

a Top: Pearson correlation coefficients between SE-regulated DEGs and MYH7 or MYH4. Bottom: Expression heatmap of SE-regulated DEGs in postnatal pig skeletal muscle at eight developmental stages. b Heatmap of 22 SE-regulated DEGs that conserved between pig and mouse SOL and EDL muscles. c Virtual 4 C plot of chromatin interactions around the STARD7 locus. Enhancers are marked in green. d 3D chromatin models of the STARD7 locus based on Hi-C data. e 3C-qPCR analysis of STARD7 promoter interactions in SOL and EDL. Interaction frequency is calculated relative to the R1 site. f Dual-luciferase reporter assay validating enhancer activity of TE-STARD7 and SE-STARD7 in PSCs (n = 3 per group). g, h Expression changes of STARD7 after TE-STARD7 (g) and SE-STARD7 (h) deletion in PSCs (n = 3 per group). For (e–h), significance was tested by a two-tailed unpaired student’s t-test. All values are presented as mean ± SEM.

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