Fig. 7: STARD7 reduces intracellular ROS levels and ERK MAPK activity. | Nature Communications

Fig. 7: STARD7 reduces intracellular ROS levels and ERK MAPK activity.

From: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation

Fig. 7

a RNA-seq tracks for STARD7, MYH4, and MYH7 loci in OV-STARD7 and empty vector (EV) PSC cells. b GO enrichment analysis of DEGs between OV-STARD7 and EV cells. c GSEA analysis of OV-STARD7 and EV cells. d Quantification of intracellular ROS levels in OV-STARD7 and EV cells (n = 3 per group). Excitation wavelength: 488 nm, emission wavelength: 525 nm. e Levels of phosphorylated ERK (p-ERK) and total ERK in OV-STARD7 and control cells (n = 3 per group). f, g qPCR and WB results showing the impact of exogenous H₂O₂ on STARD7-mediated muscle fiber-type transformation. h Schematic model of STARD7 regulation in muscle fiber-type transformation. For (d–g), significance was tested by a two-tailed unpaired student’s t-test. All values are presented as mean ± SEM.

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