Fig. 7: DRAM1 promotes the extravasation of HCC in mouse and zebrafish models. | Nature Communications

Fig. 7: DRAM1 promotes the extravasation of HCC in mouse and zebrafish models.

From: DRAM1 promotes the stability of lysosomal VAMP8 to enhance autolysosome formation and facilitates the extravasation

Fig. 7

A Representative image of Transwell migration assay (up) and transwell invasion assay (down) using HepG2 cells. Scale bar: 100 μm. B The quantification of migrated cells (upper) and invading cells (down) per field is shown in the bar graphs. Data are presented as mean ± SD from three independent experiments. C Scheme representing the metastatic assay for (D–H). D Representative lung tissue from post 5 weeks vein injection. E Bar plot showing the calculated tumor nodules in lungs, n = 5 mice. Data are shown as means ± SD representative of two independent experiments. F Representative H&E staining of lung sections post 5 weeks of injection. The arrows indicate histologically confirmed lung lesions. Scale bar: 1000 μm. G Representative macroscopic images of liver metastases via tail vein injection of 97H. The arrow indicates the visible tumor in the liver. H Representative H&E-stained liver sections. Scale bar: 1000 μm. I Scheme representing the metastatic assay. J The bioluminescence imaging of five representative mice with M3 cells injection. Luc, firefly luciferase (n = 5 mice). K Lung metastasis incidence in C57BL/6 mice after M3 injection. Data were combined from two independent experiments (n = 12 mice). L The representative image shows the immigration of 97H-mCherry in the tail region at 3 dpi. Scale bar: 100 μm. M Quantification of the number of cell clumps in the tail region by Image J, n = 9 larvae/group. N Representative image showing the extravasated 97H-GFP cells out of blood vessels in the tail region at 2 dpi. Scale bar: 100 μm. O Quantification of the percentage of extravasated 97H-GFP in the tail region. The extravasated 97H-GFP cell in the tail region was calculated by Image J. n = 9 larvae/group. Data in (A, L–O) and (D–H, J, K) are representative of three and two independent experiments, respectively. P values were calculated by two-tailed Student’s t tests (E) or One-way ANOVA with Tukey’s Multiple Comparison post hoc test (adjusted P-values for multiple comparisons) (B, M, O) and two-tailed Chi-square test for (K).

Back to article page