Fig. 6: RcCYP72H7 is responsible for catalpol formation in R. chingii. | Nature Communications

Fig. 6: RcCYP72H7 is responsible for catalpol formation in R. chingii.

From: Analysis of the Rehmannia chingii geneome identifies RcCYP72H7 as an epoxidase in iridoid glycoside biosynthesis

Fig. 6

a Genomic organization and syntenic relationships of cytochrome P450 gene clusters in R. chingii and their orthologs in S. indicum and A. thaliana. Green lines indicate the evolutionary trajectory of P450 homologs, and the red-blue gradient represents expression levels (FPKM values). b Screening of aucubin 7,8-epoxidase activity in selected P450 candidates via Agrobacterium-mediated transient expression in N. benthamiana leaves. MRM chromatograph showing aucubin (m/z 364 → 167/149) and catalpol (m/z 380 → 183/165). c Western blot analysis of N. benthamiana leaves transiently expressing RcCYP72H7-GFP, with Actin used as a loading control. d Catapol content in R. chingii leaves transiently expressing RcCYP72H7. Data are shown as mean ± SD (n = 5 biological replicates). **p < 0.01; Student’s t-test. e RcCYP72H-GFP expression in N. benthamiana leaf cells. Bar = 20 μm. Source data are provided as a Source Data file.

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