Fig. 3: SNA-L and SNA-US elicited robust antitumor immunity to suppress tumor growth in vivo.

a Experimental schedule for evaluating the antitumor immunity induced by SNA-L or SNA-US in 4T1 tumor-bearing mice in vivo. b Quantitative analysis of the frequency of CD8⁺ T cells in tumor (CD8⁺ in CD3⁺CD45⁺ cells) (n = 5 biologically independent mice). c Quantitative analysis of the frequency of IFN-γ⁺CD8⁺ T cells in tumor (IFN-γ⁺ in CD8⁺CD3⁺CD45⁺ cells) (n = 5 biologically independent mice). d Quantitative analysis of the frequency of M1-TAM in tumor (CD86⁺ in F4/80⁺CD11b⁺CD45⁺ cells) (n = 5 biologically independent mice). e Quantitative analysis of the frequency of M2-TAM in tumor (CD206⁺ in F4/80⁺CD11b⁺CD45⁺ cells) (n = 5 biologically independent mice). f Quantitative analysis of the ratio of M1-TAM to M2-TAM in tumor (n = 5 biologically independent mice). g Quantitative analysis of the frequency of Treg in tumor (CD25⁺FOXP3⁺ in CD4⁺CD3⁺CD45⁺ cells) (n = 5 biologically independent mice). h Quantitative analysis of the frequency of IL-10⁺ Treg in tumor (IL-10⁺ in CD25⁺FOXP3⁺CD4⁺CD3⁺CD45⁺ cells) (n = 5 biologically independent mice). i, Quantitative analysis of the frequency of IL-4⁺ Treg in tumor (IL-4⁺ in CD25⁺FOXP3⁺CD4⁺CD3⁺CD45⁺ cells) (n = 5 biologically independent mice). j Averaged 4T1 tumor growth curves and the photographs of the tumors after SNA or SNA-L treatment (n = 6 biologically independent mice). Scale bar = 10 mm. k Averaged 4T1 tumor growth curves and the photographs of the tumors after SNA or SNA-US treatment (n = 6 biologically independent mice). Scale bar = 10 mm. l Western blot analysis of the STING pathway activation in the idLN (n = 3 biologically independent mice). The experiment was repeated two times independently with similar results. m Schematic illustration to show SNA-L-mediated innate immune activation and immune microenvironment reprograming in the idLN. dsDNA, double stranded DNA. n GO enrichment analysis of DEG in the idLN after SNA-L treatment (n = 3 biologically independent mice). The experiment was repeated twice independently with similar results. o KEGG enrichment analysis of DEG in the idLN after SNA-L treatment (n = 3 biologically independent mice). The experiment was repeated twice independently with similar results. p Heatmap of DEG in tdLN after SNA-L treatment (n = 3 biologically independent mice). The experiment was repeated twice independently with similar results. Unless specified otherwise, the data are presented as mean ± SD. Statistical significance was calculated via one-way ANOVA (b−k) with Tukey’s test, and P-values were indicated. Source data are provided as a Source Data file. The elements in Fig. 3a were created by Adobe Illustrator, and the elements in Fig. 3m were created by Adobe Photoshop.