Fig. 1: Increases of glial-fibrillary acidic protein (GFAP) immunoreactivity in the DS and the NAc of diet-induced obese (DIO) mice.
From: Striatal astrocytes modulate behavioral flexibility and whole-body metabolism in mice
a After 90 days of the HFHS diet obese mice showed a significant increase in body weight and fat mass as compared to lean. Two-tailed unpaired Mann-Whitney test p = 0.0000007396, n = 24. b Confocal images representative of GFAP immunoreactivity (in red) in the DS and the NAc of lean (left) and DIO mice (right). c Schematic representation of the protocol. d–i In the DS and the NAc, DIO increases relative expression of GFAP immunoreactivity compared to lean ((d), DS Two-tailed unpaired t test p = 0.0536. g NAc Two-tailed unpaired t test p < 0.0015) with a positive correlation between the GFAP intensity and the fat mass content (significant Pearson correlation, (d) and (g)). It also results in a decrease of astrocytes sphericity in the DS ((e), Two-tailed unpaired t test p = 0.0075) but not in the NAc ((h), Two-tailed unpaired t test p = 0.1892). Total surface of astrocytic coverage was decreased by DIO in the DS ((f), Two-tailed unpaired t test p = 0.0030), while it was left unchanged in the NAc ((i), Two-tailed unpaired t test p = 0.2997). All data are expressed as mean ± SEM (n = 6 in each group). *p < 0.05, **p < 0.01, ***p < 0.001 ****p < 0.0001. Source data are provided as a Source Data file.
