Fig. 5: DIO-caused alteration in astrocytic Ca²⁺ activity in the NAc.

DIO increased the signal strength while decreased the temporal correlation of Ca²⁺ signals of astrocyte microdomains. a Colocalization between GCaMP6 (anti-GFP, green) and s100β (astrocyte marker, red) immunostaining in the NAc of Glast-GCaMP6f mice. b Representative pseudo-images showing regions displaying spontaneous Ca²⁺ activity in the NAc of lean (top) and obese (bottom) mice. The color bar indicates the correlation levels of the Ca²⁺ signals of the locally clustered pixels, the bright areas mirroring the multi-pixel regions showing active signals. The signal strengths of Ca²⁺ signals were derived from the temporal integral of their normalized dF/F₀ time courses (see “Methods”). Data are expressed as mean ± SEM. c Distribution of temporal correlations of spontaneous Ca²⁺ signals of paired active domains in lean (top) and obese (bottom). Right, the data of Ca²⁺ temporal correlation are expressed as mean ± SEM for the bar comparison. Lean: n = 643 active regions, 24 slices, n = 6 mice; Obese: n = 586 active regions, 19 slices, n = 4 mice. Signals were recorded for 3 min for both conditions. Source data are provided as a Source Data file.