Fig. 7: Metabolic consequences of chemogenetic activation of astrocytes of the DS after short-term exposure to energy rich diet.
From: Striatal astrocytes modulate behavioral flexibility and whole-body metabolism in mice
a Schematic representation of the HFHS exposure paradigm in C57Bl6J mice that received either AAV5-Gfap-mChery (controls) or AAV-Gfap-hM3Dq-mcherry (hM3Dq) in the DS (DSmCherry or DS-hM3Dq). Metabolic consequences of saline/CNO i.p. injection were evaluated in lean mice or after 7 days of HFHS exposure. The change in Energy expenditure (b–d and h–j) or calculated fatty acid oxidation (e–g and k–m) in response to saline (b, e, h, k) or CNO (c, f, i, l) in DSmCherry and DShM3Dq lean mice (b–g) or in the same animal group after a 7-days HFHS exposure (h−m). Mean change in Energy expenditure and fatty acid oxidation in the 5 h after Saline or CNO injection are represented per animals (2-way ANOVA, (d) treatment effect p = 0.0003, (g) treatment-group interaction p = 0.0346, (j) treatment-group interaction p = 0.0056, (m) treatment-group interaction p = 0.0040). (Lean: 2way-ANOVA EE treatment–group interaction p = 0.2118, FatOx treatment–group interaction p = 0.0346, DShM3Dq treatment factor 0.0005; 7 days: 2way-ANOVA EE treatment–group interaction p = 0.2294; FatOx treatment–group interaction p = 0.0040, DShM3Dq treatment effect p = 0.0057, group effect p = 0.0407). Multiple comparisons are performed using Šídák’s multiple comparisons test; *p < 0.05, **p < 0.01, ***p < 0.001. Graphics represent mean +/− SEM, n = 6 mice in each group. Source data are provided as a Source Data file.
