Table 1 Frequency of integration with epitope tagging using oligonucleotides

From: Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo

Allele

Number of pairs screened

Frequency of precise integration from pair incrossesa

Frequency of germline transmissionb

ALFA-nanog

8

12.5%

4%

VHH05-nanog

29

7%

12.5%

127d01-nanog

8

12.5%

17%

ALFA-vangl2

13

15.4%

25%

VHH05-vangl2

9

10.5%

7%

127d01-vangl2

17

17.7%

25%

ALFA-pou5f3

17

11.7%

12.5%

ALFA-dicer

26

7.6%

12.5%

  1. aFrequency calculated as percentage of F0 pairs that yield precisely integrate alleles as a metric for screening efficiency.
  2. bFrequency of tag positive embryos transmitted from a single fish as a metric for germline editing efficiency (from 24 randomly selected embryos).
  3. A summary of the screening for successful and precise CRISPR/Cas9 genome integration of epitope tags. Related to Figs. 3 and S6.
Back to article page