Fig. 2: Cardiomyocyte-specific Usp13 knockout aggravates cardiac hypertrophy challenged by pressure overload. | Nature Communications

Fig. 2: Cardiomyocyte-specific Usp13 knockout aggravates cardiac hypertrophy challenged by pressure overload.

From: Cardiomyocyte-derived USP13 protects hearts from hypertrophy via deubiquitinating and stabilizing STAT1 in male mice

Fig. 2

a Schematic diagram of the strategy for the generation of cardiomyocyte-specific USP13 knockout mice (USP13cKO) and the experimental timeline of TAC model (USP13cKO mice and USP13f/f mice were subjected to TAC or sham operations for 4 weeks). Myh6-Cre+/- indicates Myh6-Cre heterozygous. b Representative M-mode echocardiographic images of left ventricle were assessed by non-invasive transthoracic echocardiography. c, d M-mode echocardiographic analysis of Ejection fraction (EF) and fractional shortening (FS). e Representative images of gross-heart from each group. f The ratio of heart weight (HW) to tibial length (TL). g Representative images of heart sections stained with H&E. h, i Representative images of heart sections stained with WGA and corresponding quantitative analysis. j, k Representative Masson’s trichrome stained images of heart sections and corresponding quantitative analysis. n = 7 for each group; For (c, d, f, i, k, adjusted P values were determined by one-way ANOVA with Bonferroni’s correction and data are presented as mean ± s.e.m.

Back to article page