Fig. 2: Individual protein changes in DS by EYO.

556 out of 838 CSF proteins analyzed had different levels in DS at any EYO. Differences were assessed in 0.5 EYO intervals. Red indicates increased levels in DS and blue indicates decreased levels in DS. Heat indicates the significance of the difference between DS and controls, with scale provided in the arrows on the right. The vertical black line highlights EYO = 0 (age 50.2). Shaded EYO values on the x-axis indicate periods of lower confidence estimates, including after EYO = 10, where DS data were sparse and therefore only proteins with strong differences are considered significant. Periods of significant change and direction of change for all 556 significant proteins and other CSF ELISA measurements are provided in Supplementary Fig. 2 and Supplementary Data 2. About 266 proteins were increased and 294 proteins were decreased across EYO, with five proteins showing mixed direction of change. Visualization of proteins separated by direction of change in DS is provided in Supplementary Fig. 3. Empirical p values were computed within a Bayesian analysis framework. All tests were two-sided with a pre-specified significance level of 0.01, corresponding to tail probabilities of 0.005 and 0.995. No multiple testing correction was applied.