Fig. 4: Cryo-EM structure of EtgB. | Nature Communications

Fig. 4: Cryo-EM structure of EtgB.

From: NAD-dependent dehydrogenases enable efficient growth of Paracoccus denitrificans on the PET monomer ethylene glycol

Fig. 4

a Cryo-EM map of EtgB with the four monomers shown in different colors. Fourth monomer is not visible in this view. b Left side: front view of the structure of EtgB shown in cartoon representation, with the four monomers shown in different colors. Zinc ions are shown in blue spheres. Green trapezium shows the view in (c) and (d). Right side: top view of EtgB, rotated 90° from front view. c Close up of the active site of EtgB with residues that line the cavity in yellow sticks. Zinc ion shown in blue sphere. d Active site of P. aeruginosa alcohol dehydrogenase45 in complex with NADH and ethylene glycol (EG). Active site residues, NADH and EG shown in sticks. Zinc ion shown in blue sphere. e Surface representation of the EtgB active site, with the NADH molecule from P. aeruginosa alcohol dehydrogenase modeled into the cavity.

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