Fig. 3: The catalytic mechanism of hsGDE.
From: Molecular architecture and catalytic mechanism of human glycogen debranching enzyme
a, d The catalytic region from hsGDE GC (a) or GT (d) domains. The catalytic residues are shown in atoms. b, c and e, f Structural comparison of the catalytic region of hsGDE with glucodextranase from Arthrobacter globiformis I42 (b PDB ID:1UG9), Aspergillus oryzae alpha-Amylase (e PDB ID: 2TAA) and cgGDE (c, f PDB ID: 5D06). Glucodextranase, alpha-Amylase, and cgGDE were colored in light purple, pink, and dim gray, respectively. g−i Specific debranching activities of hsGDE and its GT/GC-defective mutants, including GC activity (g), GT activity (h), and Holoenzyme activity (i). All data in the figures indicate ratios to the wild-type value with the wild-type indicated at 1. More details about statistical analysis are shown in Supplementary Fig. 10.
