Fig. 5: Characterization of the TRAK1 Site-1 interaction with MIRO1.
From: Structural-functional characterization of the MIRO1-TRAK1 complex
a Surface representation of the MIRO11-591-TRAK1569-623 dimeric complex, highlighting the pocket (yellow) where AlphaFold337 predicts TRAK1 Site-1 (shown in all-atom representation) binds at the interface between ELM2 and cGTPase. The per-residue confidence of this prediction, as indicated by the predicted local distance difference test (pLDDT) score and the predicted aligned error (PAE), is shown in Supplementary Fig. 7a, b. A close-up view displays details of the interaction using surface and all-atom representations, with MIRO1 residue D344 (mutated to lysine to validate this interaction) shown in red. b, c ITC titrations of MBP-TRAK1416-431 into MIRO1177-591 wild-type and mutant D344K in the presence of 1 mM CaCl2 and 50 µM GTP (left) or 5 mM EGTA and 50 µM GDP (right). The experimental conditions and fitting parameters (stoichiometry, N; dissociation constant, Kd) are indicated with each graph.
