Fig. 6: MCs boost innate-like cytotoxicity of MAIT cells.

A Dot plot for the transcriptome (left) and heatmap for the surface proteome of cytotoxicity molecules and receptors (right) in MAIT cells. B Representative flow plots (left) and the percentage of (right) cytotoxicity molecules expression in MAIT cells. Each group includes 5 replicates, p values were determined by two-tailed paired Student’s t-test. C Representative flow plots (left) and the percentage of (right) NKG2D expression in MAIT cells. Each group includes 7 replicates, p value was determined by two-tailed paired Student’s t-test. D Quantification of IFN-γ secretion (left) and NKG2D expression (right) by MAIT cells with or without cytokine stimulation. Each group includes 7 replicates, p value was determined by two-tailed paired Student’s t-test. E Quantification of 4-1BB and Ki-67 expression by MAIT cells stimulated by MCs. Blue, nLung MCs, pink, LUAD MCs. Each group includes 5 replicates, p value was determined by two-tailed paired Student’s t-test. F Quantification of IFN-γ and NKG2D expression by MAIT cells stimulated by MCs. Blue, nLung MCs, pink, LUAD MCs. Each group includes 5 replicates, p value was determined by two-tailed paired Student’s t-test. G The proportion of surviving target cells GFP-A549 and H1299 cell lines by flow cytometry. Each group includes 6 replicates, p value was determined by two-tailed paired Student’s t-test. H Dot plot showing the relationship between immunotherapy response or survival and immune cell subsets using OAK cohort. I Survival analysis of MAIT cells (left) and IL-18⁺ MCs (right) in OAK cohort. p values were determined by the log-rank test. See Supplementary Fig. 6 and Supplementary Data 8.