Fig. 3: Targeting CD37 promotes phagocytosis.

A Phagocytes and macrophages were sorted from the in vitro phagocytosis assay, and CD37 was detected in phagocytes and macrophages using FACS. B The statistical analysis of CD37⁺ cells in macrophages and phagocytes as described, n = 5, data were presented as mean ± SD, two-tailed paired T-test, p < 0.0001. C Donor-derived macrophages were collected and divided into CD37-negative and -positive groups. The CD37-negative and -positive macrophages were then subjected to an in vitro phagocytosis assay to detect the phagocytic ability. D Donor-derived macrophages were labeled with cell tracker-Blue and treated with IgG or Naratuximab and then subjected to phagocytosis assay. E The statistical analysis of the percentage of phagocytosis of cells with indicated modifications, n = 3, data were presented as mean ± SD, two-tailed paired T-test, ***p < 0.0001 for all four statistical analyses. F THP1 and U937 cells were transfected with guide RNA targeting CD37 and were then induced to macrophages. Cells were collected, labeled with cell tracker-Blue, and incubated with cancer cells; the representative image of the in vitro phagocytosis assay is illustrated. G The statistical analysis of the percentage of phagocytosis of cells with indicated modifications, n = 3, data were presented as mean ± SD, paired T-test, ***p < 0.001. H Mice bearing MDA-MB-231 cancer cells were treated with IgG or Naratuximab, respectively. The representative image of the flux intensity was obtained, the overall survival time was calculated, n = 5 for each group, unpaired T-test for flux intensity, two-tailed Mantel–Cox test for overall survival analysis, p = 0.0008 for flux intensity and p = 0.0006 for overall survival analysis. I Mice bearing U251 cancer cells were treated with IgG and Naratuximab, respectively. The representative image of the flux intensity was obtained, the overall survival time was calculated, n = 5 for each group, unpaired T-test for flux intensity, two-tailed Mantel–Cox test for overall survival analysis, p = 0.0008 for flux intensity and p = 0.0007 for overall survival analysis. All the experiments were repeated at least three times with similar results.