Fig. 4: Adipocyte Cldn5 abrogation mice exhibit an obesity-prone phenotype.
From: Adipocyte CLDN5 promotes thermogenesis and energy expenditure through regulation of IL10 expression
a Body weights of Cldn5flox/flox mice (n = 12) and Cldn5flox/flox; Fabp4-Cre mice (n = 10). b Gross appearance of representative mice. c Representative image of adipose tissues. d, e Tissue weights (d) and tissue weights normalized by tibial length (e) of BAT, iWAT, and eWAT of Cldn5flox/flox mice (n = 12) and Cldn5flox/flox; Fabp4-Cre mice (n = 10). f Representative MRI images in axial and coronal planes (n = 4). A, anterior; R, right; L, left; P, posterior; S, superior; I, inferior. Scale bar, 5 mm. g HE staining with lipid droplet size quantification of BAT, iWAT, and eWAT (n = 5). h–k Energy expenditure (h), food intake (i), locomotor activity (j), and RER (k) assessed by using CalR software, n = 5. l-o qRT-PCR analysis of indicated genes related to thermogenesis and OXPHOS (l, n = 5), Western blot analysis with densitometric quantification of PGC1α and UCP1 (m, n = 3), Western blot analysis with densitometric quantification of OXPHOS proteins (n, n = 3), and mtDNA copy number (o, n = 8) in BAT. p–r qRT-PCR analysis of indicated genes related to thermogenesis and OXPHOS (p, n = 5), Western blot analysis and densitometric quantification of OXPHOS proteins (q, n = 3), and mtDNA copy number (r, n = 5) in iWAT. s Glucose tolerance test (GTT) and quantitation of AUC (n = 10). t Insulin resistance test (ITT) and quantitation of AUC (n = 8). Data are expressed as the mean ± SEM. Statistical analyses were performed using two-sided unpaired t test (a, d–g, l-t), two-sided ANCOVA (h, i), or two-sided ANOVA (j, k). AUC, area under the curve. Source data are provided as a Source Data file.
