Fig. 8: CLDN5 deficiency activates IL10 signaling to limit thermogenesis in vivo.
From: Adipocyte CLDN5 promotes thermogenesis and energy expenditure through regulation of IL10 expression
a, b Schematic of experimental design in Cldn5flox/flox; Fabp4-Cre mice, with in situ BAT (a) or iWAT (b) injections of Il10ra siRNA (si-Il10ra) or control siRNA (si-Ctrl), maintained at 4 °C for 7 days. c–g Analysis in BAT after siRNA injections: qRT-PCR analysis of Il10ra (c, n = 5), Western blot analysis with densitometric quantification of PGC1α, IL10Rα, and UCP1 (d, n = 3), qRT-PCR analysis of IL10 target genes (e, n = 5), HE staining with lipid droplet size quantification (f, n = 5), and rectal temperature (g, n = 7 for si-Ctrl, n = 9 for si-Il10ra). h-l Analysis in iWAT after siRNA injections: qRT-PCR analysis of Il10ra (h, n = 5), Western blot with densitometric quantification analyses of PGC1α, IL10Rα, and UCP1 (i, n = 3), qRT-PCR analyses of IL10 target genes (j, n = 5), HE staining with lipid droplet size quantification (k, n = 5), and rectal temperature (l, n = 5 for si-Ctrl, n = 7 for si-Il10ra). m, n Schematic of experimental design in Cldn5flox/flox; Fabp4-Cre mice, with in situ BAT (m) or iWAT (n) injections of AAV-sh-Ctrl or AAV-sh-Il10, housed for 3 weeks before 7 days at 4 °C. o-s Analysis in BAT AAV-injected mice: qRT-PCR analysis of Il10 in BAT, liver, and muscle (o, n = 6), Western blot analysis with densitometric quantification of IL10, PGC1α, and UCP1 (p, n = 3), qRT-PCR analysis of IL10 target genes (q, n = 6), HE staining with lipid droplet size quantification (r, n = 5), rectal temperature (s, n = 10). t–x Analysis in iWAT AAV-injected mice: qRT-PCR analysis of Il10 in iWAT, liver, and muscle (t, n = 6), Western blot analysis with densitometric quantification of IL10, PGC1α, and UCP1 (u, n = 3), qRT-PCR analysis of IL10 target genes in iWAT (v, n = 6 for AAV-sh-Ctrl, n = 7 for AAV-sh-Il10), HE staining with lipid droplet size quantification (w, n = 5), and rectal temperature (x, n = 10). Data are expressed as the mean ± SEM. Statistical analyses were performed using two-sided unpaired t test. Source data are provided as a Source Data file. Panels a and m were created in BioRender91. Panels b and n were created in BioRender92.
