Fig. 9: Cldn5 deletion using Ucp1-Cre and Adipoq-Cre mice recapitulates findings in Cldn5flox/flox; Fabp4-Cre mice.
From: Adipocyte CLDN5 promotes thermogenesis and energy expenditure through regulation of IL10 expression
a–e qRT-PCR analysis (a, n = 6) and Western blot analysis (b, n = 3) of CLDN5 in BAT/iWAT, rectal temperature and interscapular BAT temperature (c, n = 7), qRT-PCR analysis (d, n = 5 for Cldn5flox/flox mice, n = 6 for Cldn5flox/flox; Ucp1-Cre mice) and Western blot analysis of UCP1 (e, n = 3) in BAT/iWAT of Cldn5flox/flox and Cldn5flox/flox; Ucp1-Cre mice kept at 4 °C. f HE staining with lipid droplet size quantification in BAT/iWAT of Cldn5flox/flox and Cldn5flox/flox; Ucp1-Cre mice kept at RT or 4 °C (n = 5). g–q Body weights (g), gross appearance of representative mice (h), gross appearance, weights and weights normalized by tibial length of BAT, iWAT, and eWAT (i–k), representative MRI images shown in axial and coronal planes (l), HE staining with average lipid droplet size quantification (m, n = 5), energy expenditure (n), food intake (o), locomotor activity (p) and RER (q) in Cldn5flox/flox and Cldn5flox/flox; Ucp1-Cre mice fed HFD for 16 weeks. g, j and k, n = 13 for Cldn5flox/flox mice, n = 14 for Cldn5flox/flox; Ucp1-Cre mice. Quantification of MRI images is expressed as a percentage of total fat area over total area (n = 4). n–q, n = 5. r-t qRT-PCR analysis (r, BAT: n = 7 for Cldn5flox/flox mice, n = 6 for Cldn5flox/flox; Adipoq-Cre mice; iWAT: n = 7 per genotype) and Western blot analysis (s, n = 3) of CLDN5 in BAT/iWAT, rectal temperature, and interscapular BAT temperature (t, n = 12 for Cldn5flox/flox mice, n = 10 for Cldn5flox/flox; Adipoq-Cre mice) of Cldn5flox/flox and Cldn5flox/flox; Adipoq-Cre mice kept at 4 °C. u Body weights of the Cldn5flox/flox (n = 7) and Cldn5flox/flox; Adipoq-Cre (n = 9) mice fed a HFD for 16 weeks. Data are expressed as the mean ± SEM. Statistical analyses were performed using two-sided Mann-Whitney test (g, BAT in j and k, axial view in l, u), two-sided unpaired t test (a–f, iWAT in j and k, coronal view in l, m, r–t), two-sided analysis of ANCOVA (n, o) or two-sided ANOVA (p, q). A, anterior; R, right; L, left; P, posterior; S, superior; I, inferior. Scale bar: 5 mm (l); 100 μm (f, m). Source data are provided as a Source Data file.
